density gradient centrifugation

Methods: The selection techniques of swim-up, Percoll density gradient centrifugation and doule-flection tube were used to observe sperm chromosome abnormality rate, sex chromosomal ratio and the percentage of spermatozoa with relatively intact head ultrastructure.

方法：分别应用上游法、Percoll密度梯度离心法及双曲管优选法处理精液，观察优选前后精子染色体畸变率、精子性染色体比例及超微结构相对正常的精子百分率。

Final purification of the virus by density gradient centrifugation procedures.

通过密度梯度离心最终纯化病毒.

Conclusions The number of cells density gradient centrifugation method than with whole marrow method.

结论梯度离心法较全骨髓法收获细胞数量大.

Conclusion: High purity of MSC can be obtained by density gradient centrifugation.

结论: 密度梯度离心法分离获得的人胚胎骨髓间充质干细胞纯度高.

Objective To investigate separation and purification of skeletal muscle satellite cells with improved incontinuous density Percoll gradient centrifugation technique.

目的探讨应用改良Percoll非连续密度梯度离心法分离纯化培养组织工程用肌卫星细胞。

Six Zelanian rabbits were used to establish skin wound model, and femoral venous blood drawn before and after trauma. Mononuclear cells were purified by density gradient centrifugation. 2.

方法1.应用新西兰大白兔建立皮肤创伤模型，抽取创伤前后兔股静脉血，用密度梯度离心法分离纯化单个核细胞。

Methods: Using different techniques of collagenase digestion and discontinued density gradient centrifugation adultrat islets were prepared.

方法采用不同的胶原酶消化法及不连续密度梯度纯化法.

Purification: Density Gradient Centrifugation ( Sucrose & CsCl ).

分离: 密度梯度离心法 ( 蔗糖 & 氯化铯 ).

Mitochondria from rat liver were isolated with sucrose density gradient centrifugation, and their fatty acids were assayed by gas chromatography.

用双2乙基己基酚酞酸酯（DEHP）诱导大鼠肝过氧化物酶体增殖，然后用蔗糖密度梯度离心法分离大鼠肝线粒体，用毛细管气相色谱法测定肝线粒体中的脂肪酸含量。

Mitochondrial purification process using the two-step differential centrifugation and density gradient method.

试验方法采用多步差速离心和密度梯度离心联合法分离纯化肝脏线粒体。

Methods Rat gastric parietal cells were isolated by pronase digestion and purified by discontinuous density-gradient centrifugation.

方法采用链霉蛋白酶消化、间断密度梯度离心纯化大鼠胃黏膜壁细胞.